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KMID : 0352419980170040459
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Keimyung Medical Journal
1998 Volume.17 No. 4 p.459 ~ p.466
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Effect of Serum on Nitric Oxide Synthesis in Mouse Embryonic Liver Cells
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Hun Taeg Chung
Hyun Ock Pae/Ji Chang Yoo
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Abstract
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Serum that is commonly used as a culture medium supplement contains biologically active factors, including growth factors and cytokines. Nitric oxide (NO) is an important mediator of various biochemical and physiological processes. Its synthesis by various cells is controlled by various stimuli including cytokines or growth factors. To study the control of NO synthesis in the liver cells, the effects of serum on NO synthesis were investigated in this study. Mouse embryonic liver cell line BNL CL.2 cells were cultured 6hr in 20%, 10%, 5%, 2% serum-supplied or serum-free DMEM, respectively, and stimulated with interferon-Υ (IFN-Υ), lipopolysaccharide (LPS), or combination of two stimuli. Cells cultured in 2% serum-supplied or serum-free DMED synthesized NO in response to IFN-Υ alone in a dose- and time-dependent manner, while cells cultured in 20%, 10% or 5% serum-supplied DMEM did not. When cells were stimulated with IFN-Υ plus LPS in 2% serum-supplied or serum-free DMEM, a synergistic increase in NO synthesis was observed, whereas decrease in NO synthesis was observed in uncreasing serum concentration. These findings evidence that in vitro NO synthesis by mouse liver cells may be dependent on concentrations of serum in culture.
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KEYWORD
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Serum, Nitric oxide, BNL CL.2 cells, Liver,
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